Journal: Pain Physician

Article Title: Evaluation of the Effect of Duration on the Efficacy of Pulsed Radiofrequency in an Animal Model of Neuropathic Pain

doi: 10.36076/ppj.2018.2.191

Figure Lengend Snippet: Fig. 1. Immunopositive nerve fibers for TNF-α of CCI rats after scarification.

Article Snippet: Paraformaldehyde-fixed paraffin-embedded sciatic nerve sections (5 mm thickness) were incubated with antibodies to TNF-α (MP6-XT22, Novus Biologicals, Littleton, CO) and IL-6 (20F3, Thermo Fisher Scientific, Waltham, MA).

Techniques:

Journal: Cell reports

Article Title: Consumption of fish oil high-fat diet induces murine hair loss via epidermal fatty acid binding protein in skin macrophages

doi: 10.1016/j.celrep.2022.111804

Figure Lengend Snippet: KEY RESOURCES TABLE

Article Snippet: Rat monoclonal anti-mouse TNFα - 162Dy , Fluidigm , Cat#3162002B; RRID:AB_2801437.

Techniques: Purification, Recombinant, Activation Assay, SYBR Green Assay, Reverse Transcription, Detection Assay, Enzyme-linked Immunosorbent Assay, Selection, Software

Journal: Experimental cell research

Article Title: Using phage-assisted continuous evolution (PACE) to evolve human PD1.

doi: 10.1016/j.yexcr.2020.112244

Figure Lengend Snippet: Fig. 3. Summarization of PD1 variants that bind PDL1. (A) Under high levels of mutagenesis (MP6) during the 288 h of PACE. The Red line indicates the phage titer of each time point. And the grey line indicates the flow velocity of system. (B) During 288 h of evolution, “lagoon” samples were sequenced using the Sanger method, and 11 different preponderant oligotypes were identified which contained 9 amino acid sites. (C) Among these 11 oligotypes, 9 amino acids exhibited diverse retention from 9 times of T98L to 3 times G18A. (D) The summarization of 11 oligotypes is shown on a time axis.

Article Snippet: SP098, pAB107a, MP6 plasmid, S2060 and S2208 were obtained from Addgene.

Techniques: Mutagenesis

Journal: Blood

Article Title: Shear-induced integrin signaling in platelet phosphatidylserine exposure, microvesicle release, and coagulation

doi: 10.1182/blood-2017-05-785253

Figure Lengend Snippet: Inhibition of shear-dependent platelet MV release and PS exposure in mouse and human platelets by mP6, a peptide inhibitor of Gα13-integrin interaction and outside-in signaling. (A) Effects of mP6 or a negative control peptide (AAA mutant, Myr-FAAARA) on MV release from mouse platelets stimulated by 0.05 U/mL of thrombin with shear of 3000 s−1 as analyzed by flow cytometry. (B) Effects of mP6 or a control peptide on PS exposure on mouse platelets stimulated by 0.05 U/mL of thrombin with shear of 3000 s−1 as indicated by annexin V binding. (C) Effects of mP6 or a control peptide on MV release from human platelets stimulated with 0.05 U/mL of thrombin with or without shear. (D) Effects of mP6 (40 µM) or a control peptide on PS exposure on human platelets stimulated with 0.05 U/mL of thrombin with or without shear. (E) MV release from thrombin- and shear-stimulated mouse platelets treated with dimethyl sulfoxide (DMSO; vehicle control), Src inhibitor PP2, integrin antagonist integrilin, or Rac1 inhibitor NSC23766. (F) PS exposure on thrombin- and shear-stimulated platelets treated with DMSO, PP2 (10 µM), integrilin (20 µg/mL), or NSC23766 (100 µM). (G) MV release from CRP- and shear-stimulated platelets treated with DMSO, PP2, integrilin, or NSC23766. (H) PS exposure on CRP- and shear-stimulated platelets treated with DMSO, PP2, integrilin, or NSC23766. Data are presented as mean ± standard error of the mean (n = 3-4). **P < .01, ***P < .001, and ****P < .0001.

Article Snippet: Myristoylated peptides mP6 (Myr-FEEERA) and the corresponding control peptides, mP6Scr (Myr-ERAFEE) and AAA (Myr-FAAARA), were synthesized and purified at the Research Resource Center at the University of Illinois at Chicago or provided by Dupage Medical Technology, Inc. (Willowbrook, IL).

Techniques: Inhibition, Shear, Negative Control, Mutagenesis, Flow Cytometry, Control, Binding Assay

Journal: Blood

Article Title: Shear-induced integrin signaling in platelet phosphatidylserine exposure, microvesicle release, and coagulation

doi: 10.1182/blood-2017-05-785253

Figure Lengend Snippet: BFP analysis of integrin-dependent transduction of mechanical force leading to PS exposure and in vitro analysis of the role of integrin outside-in signaling in PPA. (A) Integrin ligand FN-conjugated BFP was allowed to repeatedly touch and pull the membrane surface of a resting or thrombin-stimulated platelet treated with or without mP6 or a scrambled control peptide (mP6Scr). Annexin V binding frequency to the same platelet was then assessed using a second BFP conjugated with annexin V (supplemental Figure 1). Data are shown as median (center bars) ± interquartile range (boxes) and maximal/minimal range (range bars). Note that annexin V binding to thrombin-stimulated platelets was significantly enhanced by frequent pulling applied through FN-coated BFP, which was inhibited by mP6 but not by mP6Scr. (B) The recalcification-induced clotting time (mean ± standard error of the mean [SEM]) of citrated human platelet-depleted plasma reconstituted with washed wild-type (WT) or β3−/− mouse platelets (WT, n = 6; β3−/−−, n = 8) under stirring conditions. (C-D) The recalcification-induced clotting time (mean ± SEM) of human citrated platelet-rich plasma treated with 40 µM of mP6 or a control peptide was monitored under stirring conditions in a turbidometric platelet aggregometer (C) (n = 9) or detected using a cone-and-plate rheometer under shear rate of 6000 s−1 (D) (n = 5). *P < .05, **P < .01, and ****P < .0001. N.S., not significant.

Article Snippet: Myristoylated peptides mP6 (Myr-FEEERA) and the corresponding control peptides, mP6Scr (Myr-ERAFEE) and AAA (Myr-FAAARA), were synthesized and purified at the Research Resource Center at the University of Illinois at Chicago or provided by Dupage Medical Technology, Inc. (Willowbrook, IL).

Techniques: Transduction, In Vitro, Membrane, Control, Binding Assay, Coagulation, Clinical Proteomics, Shear

Journal: Blood

Article Title: Shear-induced integrin signaling in platelet phosphatidylserine exposure, microvesicle release, and coagulation

doi: 10.1182/blood-2017-05-785253

Figure Lengend Snippet: The effect of mP6 on fibrin generation and platelet thrombus formation in vivo using laser-induced mouse arteriolar thrombosis model. Intravital microscopy was used to monitor fibrin generation and platelet thrombi in vivo after laser-induced cremaster arteriole wall injury in wild-type mice treated with or without selective outside-in signaling inhibitor mP6 (10 µmol/kg) or scrambled control peptide (mP6Scr; 10 µmol/kg). The median integrated (Int.) fluorescence (FL) signals of fibrin (A) and platelets (B) from 28 thrombi in mP6-treated mice, 26 thrombi in mP6Scr-treated mice, or 27 thrombi in untreated mice are shown as a function of time. The median of the total FL detected over time for fibrin (C) or platelets (D) is shown, calculated by integrating the area under the curve. (E) Representative images of fibrin generation (green) and platelet thrombi formation (red) and merged images. ****P < .0001.

Article Snippet: Myristoylated peptides mP6 (Myr-FEEERA) and the corresponding control peptides, mP6Scr (Myr-ERAFEE) and AAA (Myr-FAAARA), were synthesized and purified at the Research Resource Center at the University of Illinois at Chicago or provided by Dupage Medical Technology, Inc. (Willowbrook, IL).

Techniques: In Vivo, Intravital Microscopy, Control, Fluorescence